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sta21  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology sta21
    Sta21, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sta21/STA-21/pm35820245-47-0-4
    Average 93 stars, based on 44 article reviews
    sta21 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Cell Culture:

    Article Title: The Therapeutic Effect of STAT3 Signaling-Suppressed MSC on Pain and Articular Cartilage Damage in a Rat Model of Monosodium Iodoacetate-Induced Osteoarthritis
    Article Snippet: Cells were resuspended and cultured with Dulbecco's modified Eagle's medium containing 10% fetal bovine serum (Pan-Biotech, Aidenbach, Germany). .. The cells were cultured for 4 days until 90% confluent (passage 0) and were then expanded for 2–3 passages and used for experiments. iSTAT3 OA-MSCs were prepared by treatment of OA-MSCs with 10 μM STA21 (Santa Cruz, Texas, USA) for 72 h. ..

    Transduction:

    Article Title: ADAM9 enhances Th17 cell differentiation and autoimmunity by activating TGF-β1
    Article Snippet: In addition to Th0-nonpolarizing conditions, the following stimulations were used for each polarizing condition: IL-12 (20 ng/mL, R&D Systems) and anti-IL-4 (10 μg/mL, C17.8; Bio X Cell) for Th1 condition; IL-4 (100 ng/mL, R&D Systems) and anti-IFNγ (10 μg/mL, XMG1.2; Bio X Cell) for Th2 condition; IL-6 (3 ng/mL, R&D Systems), TGF-b1 (0.3 ng/mL, R&D Systems), anti-IL-4 (10 μg/mL) and anti-IFNγ (10 μg/mL) for Th17; IL-6 (3 ng/mL), latent TGF-b1 (1.0 ng/mL, R&D Systems), anti-IL-4 (10 μg/mL) and anti-IFNγ (10 μg/mL) for Th17 with latent TGF- β1 condition; and IL-2 (20 ng/mL, R&D Systems), TGF-β1 (1.0 ng/mL, R&D Systems), anti-IL-4 (10 μg/mL) and anti-IFNγ (10 μg/mL) for Tregs condition; and IL-2 (20 ng/mL), latent TGF-β1 (3.0 ng/mL), anti-IL-4 (10 μg/mL) and anti-IFNγ (10 μg/mL) for Tregs with latent TGF-β1 condition. .. For signal transduction studies, STA21 (STAT3 inhibitor, Santa Cruz Biotechnology) was added to cultures on day 0. .. For the human Th17-polarized culture, isolated naive CD4 + T cells in RPMI medium 1640 with 10% fetal bovine serum plus penicillin-streptomycin were stimulated with plate-bound anti-CD3 Ab (1 μg/mL, OKT-3; Bio X Cell), anti-CD28 Ab (1 μg/mL, CD28.2; BioLegend), IL-6 (50 ng/mL, BioLegend), TGF-β1 (10 ng/mL, BioLegend), IL-1β (10 ng/mL, BioLegend), IL-23 (50 ng/mL, BioLegend), anti-IL-4 Ab (10 μg/mL, Bio X Cell), and anti-IFN-γ Ab (5 μg/mL, B27; BioXCell) at 37 °C.

    Binding Assay:

    Article Title: STAT3 Phosphorylation Mediating DMSO's Function on Fetal Cardiomyocyte Proliferation with Developmental Changes.
    Article Snippet: Ventricles were enzymatically dissociated to get single cells as previously described.24-26) The isolated cells were plated on sterile gelatin-coated glass cover slips, cultured in Dulbecco’s Modified Eagle’s Medium (Gibco) containing 20% fetal bovine serum (Gibco), and kept in the incubator for 24 hours for further treatment according to the following experiments. .. DMSO (from 1:100,000 to 1:1000, Sigma, USA), STA21 (selective STAT3 DNA binding inhibitor) (Santa Cruz Biotechnology, USA) or BrdU (0.03 mg/mL) (Boster, China) was applied where necessary. ..

    Inhibition:

    Article Title: Characterization of wild-type and STAT3 signaling-suppressed mesenchymal stem cells obtained from hemovac blood concentrates
    Article Snippet: .. Inhibition of STAT3 signaling in MSCs (iSTAT3-MSCs) iSTAT3-MSCs were obtained by adding 10 μM STA21 (Santa Cruz Biotechnology, Dallas, TX, USA) to OA-MSCs for 72 h. Enzyme-linked immunosorbent assay (ELISA) The concentrations of IL-6 (DY206-05; R&D Systems, Minneapolis, MN, USA), IL-8 (DY206, RRID: AB_2814717; R&D Systems), IL-1β (DY201-05; R&D Systems), IL-10 (DY217B-05; R&D Systems), TGF-β (DY240-05; R&D Systems), and vascular endothelial growth factor (VEGF; DY293B-05; R&D Systems) were measured in the culture supernatants of OA-MSCs and iSTAT3-MSCs using commercial ELISA kits, according to the manufacturer’s instructions. .. Flow cytometry The surface markers of OA-MSCs and iSTAT3-MSCs were analyzed using a FACSCalibur Flow Cytometer (BD Biosciences, San Jose, CA, USA).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Characterization of wild-type and STAT3 signaling-suppressed mesenchymal stem cells obtained from hemovac blood concentrates
    Article Snippet: .. Inhibition of STAT3 signaling in MSCs (iSTAT3-MSCs) iSTAT3-MSCs were obtained by adding 10 μM STA21 (Santa Cruz Biotechnology, Dallas, TX, USA) to OA-MSCs for 72 h. Enzyme-linked immunosorbent assay (ELISA) The concentrations of IL-6 (DY206-05; R&D Systems, Minneapolis, MN, USA), IL-8 (DY206, RRID: AB_2814717; R&D Systems), IL-1β (DY201-05; R&D Systems), IL-10 (DY217B-05; R&D Systems), TGF-β (DY240-05; R&D Systems), and vascular endothelial growth factor (VEGF; DY293B-05; R&D Systems) were measured in the culture supernatants of OA-MSCs and iSTAT3-MSCs using commercial ELISA kits, according to the manufacturer’s instructions. .. Flow cytometry The surface markers of OA-MSCs and iSTAT3-MSCs were analyzed using a FACSCalibur Flow Cytometer (BD Biosciences, San Jose, CA, USA).



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    Figure 5 |LPS-activated microglia promote astrocyte proliferation in vitro. (A) EdU staining and cellular localization with astrocytes cocultured under different conditions. (B) Western blotting analysis of <t>STAT3,</t> pSTAT3 and GFAP in the mixed cells after 24 hours of incubation. (C, D) The quantification of pSTAT3 (C, n = 3) and GFAP (D, n = 3) was normalized to β-actin. (E) LPS-activated microglia significantly increases the proportion of EdU+/GFAP+ astrocytes, which was reversed by <t>STA21</t> (an inhibitor of STAT3) pretreatment (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (one-way analysis of variance with Tukey’s post hoc test). Data are expressed as the mean ± SD. Western blot experiments were repeated three times. Immunofluorescence staining was repeated six times. DAPI: 4′,6-Diamidino-2-phenylindole; EdU: 5-ethynyl-2-deoxyuridine; GFAP: glial fibrillary acidic protein; LPS: lipopolysaccharide; pSTAT3: phosphorylated STAT3; SCI: spinal cord injury; STAT3: signal transducers and activators of transcription 3.
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    Figure 5 |LPS-activated microglia promote astrocyte proliferation in vitro. (A) EdU staining and cellular localization with astrocytes cocultured under different conditions. (B) Western blotting analysis of <t>STAT3,</t> pSTAT3 and GFAP in the mixed cells after 24 hours of incubation. (C, D) The quantification of pSTAT3 (C, n = 3) and GFAP (D, n = 3) was normalized to β-actin. (E) LPS-activated microglia significantly increases the proportion of EdU+/GFAP+ astrocytes, which was reversed by <t>STA21</t> (an inhibitor of STAT3) pretreatment (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (one-way analysis of variance with Tukey’s post hoc test). Data are expressed as the mean ± SD. Western blot experiments were repeated three times. Immunofluorescence staining was repeated six times. DAPI: 4′,6-Diamidino-2-phenylindole; EdU: 5-ethynyl-2-deoxyuridine; GFAP: glial fibrillary acidic protein; LPS: lipopolysaccharide; pSTAT3: phosphorylated STAT3; SCI: spinal cord injury; STAT3: signal transducers and activators of transcription 3.
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    Image Search Results


    Figure 5 |LPS-activated microglia promote astrocyte proliferation in vitro. (A) EdU staining and cellular localization with astrocytes cocultured under different conditions. (B) Western blotting analysis of STAT3, pSTAT3 and GFAP in the mixed cells after 24 hours of incubation. (C, D) The quantification of pSTAT3 (C, n = 3) and GFAP (D, n = 3) was normalized to β-actin. (E) LPS-activated microglia significantly increases the proportion of EdU+/GFAP+ astrocytes, which was reversed by STA21 (an inhibitor of STAT3) pretreatment (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (one-way analysis of variance with Tukey’s post hoc test). Data are expressed as the mean ± SD. Western blot experiments were repeated three times. Immunofluorescence staining was repeated six times. DAPI: 4′,6-Diamidino-2-phenylindole; EdU: 5-ethynyl-2-deoxyuridine; GFAP: glial fibrillary acidic protein; LPS: lipopolysaccharide; pSTAT3: phosphorylated STAT3; SCI: spinal cord injury; STAT3: signal transducers and activators of transcription 3.

    Journal: Neural regeneration research

    Article Title: Microglial depletion impairs glial scar formation and aggravates inflammation partly by inhibiting STAT3 phosphorylation in astrocytes after spinal cord injury.

    doi: 10.4103/1673-5374.357912

    Figure Lengend Snippet: Figure 5 |LPS-activated microglia promote astrocyte proliferation in vitro. (A) EdU staining and cellular localization with astrocytes cocultured under different conditions. (B) Western blotting analysis of STAT3, pSTAT3 and GFAP in the mixed cells after 24 hours of incubation. (C, D) The quantification of pSTAT3 (C, n = 3) and GFAP (D, n = 3) was normalized to β-actin. (E) LPS-activated microglia significantly increases the proportion of EdU+/GFAP+ astrocytes, which was reversed by STA21 (an inhibitor of STAT3) pretreatment (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (one-way analysis of variance with Tukey’s post hoc test). Data are expressed as the mean ± SD. Western blot experiments were repeated three times. Immunofluorescence staining was repeated six times. DAPI: 4′,6-Diamidino-2-phenylindole; EdU: 5-ethynyl-2-deoxyuridine; GFAP: glial fibrillary acidic protein; LPS: lipopolysaccharide; pSTAT3: phosphorylated STAT3; SCI: spinal cord injury; STAT3: signal transducers and activators of transcription 3.

    Article Snippet: To assess the role of STAT3 signaling, astrocytes were pretreated with 10 μM STA21 (an inhibitor of STAT3, Selleck, Shanghai, China, Cat# S7951) for 72 hours before coculture.

    Techniques: In Vitro, Staining, Western Blot, Incubation, Immunofluorescence